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�й���������־ 2009, 34(11) 659- DOI:   1001-8689��2009��11-0659-05  ISSN: 0412-1961 CN: 21-1139/TG

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Article by Z. S. L.
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Effects of the dissolved oxygen on the erythromycin components of recombinant strain Saccharopolyspora erythraea ZL1004 fermentation
State Key Laboratory of Bioreactor Engineering ECUST, Shanghai 200237
Abstract:

Effects of the oxygen supply on the erythromycin components of recombinant strain Saccttaropolyspora erythraea EL1004 fermentation were investigated. The oxygen supply was changed by using different kinds of shakers or varying volume of fermentation broth in the same shake flask. The results showed that the compoments of erythromycin were improved when the fermentation was carried out in the opened rotary shaker, the ratio of erythromycin A reached 92.93% while the byproducts erythromycin B was 4.59% and erythromycin C was 1.48% at the end point of the fermentation. The least fermentation volume of 35ml in the 500ml flask produced the best fermentation composition�� erythromycin A was 92.13%, erythromycin B was 1.73%, and erythromycin C was 6.14% ��. A further study in a 50L fermenter demonstrated that erythromyein B was completely eliminated when the dissolved oxgen was kept above 40%. In comparison with the low oxygen supply �� the dissolved oxgen was kept below 40% ��, the content of erythromycin A was increased by 13.51% while erythromycin C was decreased around 71.3%. Taken together, our results showed that the control of dissolved oxygen was important to improve the ratio of erythromycin components in the recombinant strain S. erythraea ZL1004 fermentation.

Keywords: Erythromycin    Components    Recombinant strains    Hydroxylase    Eryk  
�ո����� 2009-03-27 �޻�����  ����淢������  
DOI: 1001-8689��2009��11-0659-05
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