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�й�������־ 2009, 34(11) 688- DOI:   1001-8689��2009��11-0688-03  ISSN: 0412-1961 CN: 21-1139/TG

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PubMed
Article by Fang, X. Q.
Article by L. Y. N.
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Synergism of roxythromycin and imipenem to Pseudomonas aeruginasa in biofilm
Department of Pulmonary Medicine of Chinese PLA General Hospital, Beijing 100853
Abstract:

Objective The influence of roxythrgmycin ��RXM�� on the production of glycocalyx ��GLX�� from P. aeruginosa biofilms and synergism of antibacterial activities of RXM and imipenem ��IMI�� to P. aerugirtasa in biofilms was investigated. Methods GLX production was measured by using a L-tryptophan method and viable counts in biofilms was determined by using a methylthiazolyhetrazolium ��MTT�� method Results GLX production was reduced significantly from 32.5 ± 15.7 μg/ml at 0MIC to 24.3 ± 13.3 μg/ml at 1/16MIC and 18.6 ± 9.5μg/ml at 1/4MIC respectively �� P �� 0.05 ��. 1/16MIC and 1/4MIC RXM showed no bactericidal activities to P. aeruginosa in biofilms. Viable counts in biofilms were 6.8 ±0.2LgCFU/cm2 at 0MIC, 6.7 ±0.2LgCFU/cm2 at 1/16MIC and 6.7 ± 0. 3LgCFU/cm2 at 1/4 MIC respectively ��P ��0.05 ��. However, RXM could enhance bactericidal activity of IMI to P. aeruginosa in biofilms. When 1/4MIC IMI was combined with I/4MIC and 1/16MIC RXM, viable counts decreased significantly from 6.7 ± 0.3 LgCFU/cm2 to 6.3 ,± O. 4 LgCFU/cm2 and 6.1 ± 0.5 LgCFU/cm2 respectively �� P �� 0.05 ��. When 1/2MIC IMI was combined with 1/4MIC and 1/16MIC RXM, viable counts decreased significantly from 6.6 ± 0.3 LgCFU/cm2 to 6. 3 ± 0.5LgCFU/cm2 and 6. 1 ± 0.4LgCFU/cm2 respectively �� P �� 0.05 ��, When 1MIC IMI was combined with 1/4MIC and 1/16MIC RXM, viable counts decreased significantly from 6.4 ±0.5LgCFU/cm2 to 5.6 ± 0.8LgCFU/cm2 and 5.0 -± 0.7LgCFU/cm2 respectively �� P �� 0.05 ��. Conclusion Since RXM did not have direct bactericidal activity to P. aeruginosa in biofilms but could inhibit GLX production, we considered that RXM could enhance antibacterial activity of IMI by enhancing permeability of IMI into biofilms.

Keywords: Biofilm     Glycocalyx   Roxythromycin    Imipenem  
�ո����� 2009-04-01 �޻�����  ����淢������  
DOI: 1001-8689��2009��11-0688-03
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