Abstract：Objective To establish the microbial limit test of different dosage forms of voriconazole by different pretreatment methods. Methods According to the physical and chemical characteristics of the capsule, powder and cream, the microbial limit test methods of different preparation of voriconazole were established by plating and membrane filtration, and the differences were compared. Results The microbial limit test methods of three different formulations of voriconazole met the requirements of Chinese Pharmacopoeia 2015, the test microorganisms recovery rates of total aerobic microbial count and total combined yeasts and molds count were 50%~200%, and the added specified microorganisms were checked out. The methods can be used for microbiological examination of miconazole nitrate. Conclusion The methods of the microbial limit test of different dosage forms of miconazole nitrate are different, and the suitable pretreatment method should be flexibly selected to solve the problem of antibacterial removal and filtration.