Abstract：Objective To investigate molecular epidemiology and mechanisms of clinical isolates of Klebsiella pneumoniae resistant to carbapenems. Methods Antimicrobial susceptibility test was done on 24 strains of Klebsiella pneumoniae by the Kirby-Bauer method. The minimal inhibitive concentrations (MICs) of the antimicrobial agents were determined by the microdose broth dilution method. A modified Hodge test may assist in confirming the presence of carbapenemase. The genotype of KPC, IMP, NDM, VIM, IMI, SPM, OXA-23, OXA-24, OXA-48, and OXA-58 was detected by the polymerase chain reaction (PCR). Results Clinical isolates of carbapenem-resistant Klebsiella pneumoniae were multi-drug resistant. Positive rate of blaIMP in Klebsiella pneumoniae was 58.3% (It was later detected as IMP-4). But no KPC, NDM, VIM, IMI, SPM, OXA-23, OXA-24, OXA-48, and OXA-58 genes were detected. Conclusion Production of metallo-β-lactamase IMP-4 in Klebsiella pneumoniae was the main mechanisms of carbapenem resistance.