Abstract：Objective To collect carbapenem-resistant Enterobacteriaceae (CRE) strains from five hospitals in
Hainan and analyze their drug resistance genes and prevalence features. Methods CRE strains were collected from
inpatients clinical samples from June 2014 to June 2016. Bacteria identification and drug resistance were analyzed
by Bio-Merieux VITEK-2 Compact identification system. The drug resistance genes of KPC-2, NDM-1and IPM
were amplified by PCR, and then the products were analyzed by agarose gel electrophoresis and sequenced. The drug
resistance genotypes of CRE strains were determined by BLAST. Results 75 CRE strains were collected from the
five hospitals. In 75 CRE strains, 45 were Klebsiella pneumoniae (60%), 13 were Enterobacter cloacae (17.3%).
43.9% collected samples were from ICU and 10.7% were from pediatric. The resistance rates of 75 CRE strains
were listed as follows: prostacyclin (10.6%), amikacin (23.3%), levofloxacin (37.3%), ciprofloxacin (40%), the first
to third generation cephalosporins (100%), ampicillin (100%), ampicillin/shubatan (100%), the fourth generation
cephalosporin cefepime (89.3%), SMZ-TMP (78.7%), and piperacillin/tazobactam (64%). Among the 75 CRE strains, 11 were blaKPC-2 positive (14.6%), 25 were blaNDM-1 positive (33.3%), and 12 were blaIMP positive (16%). Conclusion
blaNDM-1 is the major genotype of CRE in Hainan, and blaKPC-2 is firstly reported in this study. The drug resistance
mechanism of CRE should be further studied for strains without the three genes.
苏屿 李天娇* 龙文芳 黄林娇 符生苗 黄涛 符惠群. 海南耐碳青霉烯类肠杆菌科细菌的耐药基因分析[J]. 中国抗生素杂志, 2018, 43(9): 1156-1160.
Su Yu,Li Tian-jiao,Long Wen-fang,Huang Lin-jiao,Fu Sheng-miao,Huang Tao and Fu Hui-qun. Analysis of drug resistance genes of carbapenem resistant
Enterobacteriaceae in Hainan province. CJA, 2018, 43(9): 1156-1160.
