Abstract：Objective To develop the separation and purification processes of lipiarmycin. Methods Using a binary liquid chromatography system, the separation and purification processes of lipiarmycin were studied by optimizing the different types of chromatography fillers and elution conditions to obtain a suitable operating conditions. Results The results showed that the type of reverse phase silica gel C18 was selected as the best chromatography filler, 50% acetonitrile-H2O was used as the eluent, and the amount of sample was 10mg/mL filter . The purity of the final product was more than 98.5% and the yield of lipiarmycin was above 70% under the optimal conditions. Conclusion The method is simple and available for the commercial process.
高月麒 李晓露 王海燕 王娅莉 林旸 任风芝* 张雪霞*. 闰年霉素分离纯化工艺研究[J]. 中国抗生素杂志, 2018, 43(12): 1493-1497.
Gao Yue-qi, Li Xiao-lu, Wang Hai-yan, Wang Ya-li, Lin Yang, Ren Feng-zhi and Zhang Xue-xia. Studies on the separation and purification of lipiarmycin. CJA, 2018, 43(12): 1493-1497.