Abstract：Objective To investigate the carrying status and drug sensitivity of pharyngeal colonized bacteria in healthy preschoolers, and provide theoretical basis for rational drug use in clinic. Methods The results of bacterial culture and drug sensitivity of pharyngeal swab specimens were analyzed in 119 healthy preschoolers who were placed in a public kindergarten for the first time in urban area. Results Among 119 healthy preschoolers, there were up to five kinds of pharyngeal colonization bacteria and zero kinds at least, with an average of (3.36+0.79) kinds. Among the 108 children, 135 strains of medium (++) bacteria were isolated from semi-quantitative culture, mainly including Streptococcus viride, Moraxella catarrhalis, Streptococcus pneumoniae, Staphylococcus aureus, and Haemophilus influenzae. Drug susceptibility test showed that colonized bacteria had a high resistance rate to erythromycin, tetracycline, compound trimethoprim, clindamycin, and other commonly used antibiotics in clinic. Conclusion The pharyngeal colonization bacteria of healthy preschoolers in this area were mainly conditional pathogenic bacteria, some of which were mixed colonization. They were highly resistant to commonly used antibiotics in clinic. It is very important to standardize the use of antibiotics in children with upper respiratory tract infections.