Abstract：Abstract Objective To investigate secondary metabolites produced by a deep-sea derived fungus, Aspergillus
sp. 20220129 isolated from the sediment sample of the western Pacific Ocean. Methods The secondary metabolites
were isolated and purified by silica gel column chromatography and high performance liquid chromatography. Their
chemical structures were identified by analysis and comparison of spectroscopic data from MS, 1H NMR, and 13C
NMR. Antimicrobial activities of these compounds were evaluated by the microplate dilution method. Results Nine compounds were isolated and identified from the rice solid fermentation materials. These compounds were terretonin
(1), terretonin A(2), methyl dichloroasterrate (3), methyl asterrate (4), methyl 6-acetyl-5,7,8-trihydroxy-4-methoxy-
2-naphthalenecarboxylate (5), territrem B (6), alantrypinone (7), butyrolactone I (8), and versicolactone B (9).
Compounds 5, 8, and 9 displayed antibacterial activities against S. aureus with minimum inhibitory concentration
values of 100, 100, and 50 μg/mL, respectively.
