Abstract：Abstract Objective To study the secondary metabolites and their activities from a marine-derived fungus Aspergillus sp. WHUF0313. Methods The silica gel column chromatography, SephadexLH-20 gel column chromatography, and high-performance liquid chromatography (HPLC) were used to isolate and purify the secondary metabolites from the fermented extracts of the fungus Aspergillus sp. WHUF0313. Their structures were elucidated
on the basis of Nuclear Magnetic Resonance analyses (NMR) and Mass Spectrum (MS) spectroscopic data and by
comparison with the data of literatures. Their antibacterial and cytotoxic activities were evaluated by microdilution
assay and MTS assay, respectively. Results Nine compounds were isolated and identified as asperophiobolin G
(1), asperophiobolin F (2), ophiobolin Q (3), asperophiobolin H (4), 6-epi-ophiobolin G (5), ophiobolin H (6), 6-epiophiobolin
K (7), 6-epi-21,21-O-dihydroophiobolin G (8) and asperophiobolin J (9). Conclusion Many bioactive
compounds were produced by the marine-derived fungus Aspergillus sp. WHUF0313. Compound 4 exhibited
cytotoxic activity against malignant melanoma B16 cell lines. Compounds 6 and 7 exhibited antibacterial activities
against multi-drug resistant strains Helicobacter pylori and Staphylococcus aureus. The fungus Aspergillus sp.
WHUF0313 has potential research value in the development of microbial drugs.
王森1 洪葵2 霍瑞云3 贾佳4 孙立彦1,* 刘玲3,*. 1株海洋真菌Aspergillus sp. WHUF0313次级代谢产物及其活性研究[J]. 中国抗生素杂志, 2022, 47(05): 507-513.
Wang Sen1, Hong Kui2, Huo Rui-yun3, Jia Jia4, Sun Li-yan1, and Liu Ling3. Bioactive secondary metabolites from a marine-derived fungus Aspergillus sp. WHUF0313. CJA, 2022, 47(05): 507-513.
