Abstract：Abstract Objective To mine secondary metabolite biosynthesis gene clusters of Streptomyces sp. V-1-3, predict its metabolites and lay the foundation for the discovery of new antibiotics. Methods The 16S rRNA gene sequencing was employed to preliminarily identify the strain. Streptomyces sp. V-1-3 was sequenced by Illumina HiSeq + PacBio sequencing technology to mine secondary metabolite by antiSMASH (v6.0.1) online tool and the secondary metabolites of Streptomyces sp. V-1-3 were further analyzed by HPLC-Q-TOF MS. Results The size of the genome was 8,243 417 bp with an average of G+C content at 72.14 %, which had 7,578 genes. Biosynthetic gene clusters for thirty-three secondary metabolites were predicted. Four compounds, oxalomycin B, geosmin, coelichelin, and ishigamide were validated by HPLC-Q-TOF MS analysis. Conclusion Streptomyces sp. V-1-3 from saline-alkali soil has abundant secondary metabolite biosynthesis gene clusters, and can produce different types of secondary metabolites, which has the value for further exploring to find new antibiotics.
胡运琪1 马晓莉1 贾荣亮2 林文星3 郭欢欢1 李彬4 李舂龙1 南泽东1 吴秀丽3 江志波1,*. 宁夏枸杞根际土壤链霉菌V-1-3次级代谢产物分析[J]. 中国抗生素杂志, 2022, 47(07): 647-653.
Hu Yun-qi1, Ma Xiao-li1, Jia Rong-liang2, Lin Wen-xing3, Guo Huan-huan1, Li Bin4, Li Chong-long1, Nan Ze-dong1, Wu Xiu-li3, and Jiang Zhi-bo1. Study on secondary metabolites of Streptomyces sp. V-1-3 from the rhizosphere soil of Lycium barbarum in Ningxia. CJA, 2022, 47(07): 647-653.
