Abstract：Objective To evaluate the quality of triamcinolone acetonide and neomycin paste from different manufactures. Methods The quality and the standard controllability of triamcinolone acetonide and neomycin paste were evaluated by researching on the original standard and the exploratory experiments including the improvement of the identification method of triamcinolone acetonide acetate and neomycin sulfate, assay of triamcinolone acetonide acetate and neomycin sulfate, and the determination of the content uniformity and the residual solvents. Results The qualified rate was 98.9% for the 267 batches of samples extracted according to the original standard. The results of exploratory research showed that the improved identification method was specific, accurate, and well operated, which could identify the two principal components well. There were big differences for the assay results among different manufacturers, where five were unqualified among the six manufactures and the homogeneity were poor meanwhile; the residual solvents exceeded the specified limit for one of the manufacturers. Conclusion The quality of triamcinolone acetonide and neomycin paste was generally not bad and the current standard should be further improved. It was proposed that the identification methods for the two principal components should be improved and the method for the assay of triamcinolone acetonide and neomycin sulfate should be added. However, it was necessary to optimize the production process, control the uniformity of the content of the product and the residual solvents for better quality control.