Abstract：Objective To investigate the distribution and the antibiotic resistance of carbapenem resistant Enterobacteriaceae (CRE). Methods The detection, infection characteristics and drug resistance of CRE isolates were evaluated by the Phoenix-100 automatic bacterial identification/drug sensitivity system, the K-B method, the modified Hodge test, and the EDTA synergy test. Results A total of 1,020 clinical isolates of CRE were isolated from the clinical specimens of patients in our hospital. The detection rate of CRE was 5.5%, and the positive rate of the modified hodge test was 96. 6% (985/1,020). The annual detection rates of CRE from 2013 to 2017 were 0.8% (24/2,858), 8.6% (271/3,156), 5.9% (249/4205), 6.2% (245/3,935), and 5.5% (231/4,293), respectively. 1,020 clinical isolates of CRE mainly included 988 isolates (96.8%) of Klebsiella pneumoniae, among which 779 isolates were from respiratory tract specimens (76.2%), 87 isolates were from blood specimens (8.4%) and 45 isolates were from catheter tips (4.5%), 478 isolates were from the ICU department (46.9%) and 315 isolates were from NICU (30.8%). Drug sensitivity test results showed that the resistance rates of CRE to gentamicin, amikacin, cotrimoxazole, polymyxin and tigecycline were 53.2%, 39.4%, 45.9%, 0 and 0, respectively. The resistance rate of CRE to other commonly used antibiotics was higher than 97.5%. The resistance rate to gentamicin decreased from 80% to 48.3%. The drug resistance rate of CRE to cotrimoxazole increased from 33.3% to 60%. The CRE was sensitive to polymyxin and tigecycline. So far, our hospital has not yet found the CRE which was resistant to polymyxin and tegicycline. Conclusion The isolation rate of CRE was increasing year by year, in which carbapenem-resistant Klebsiella pneumoniae was the main isolates. The prevalence of CRE in our hospital was mainly isolated from the ICU and NICU, and thus we should investigate the risk factors of CRE infections and take effective measures to control infections.