Abstract：Objective To identify the antibacterial secondary metabolites produced by Aspergillus fumigatus GZWMJZ-152, a fungus from a cave in Mount Fanjing in Guizhou. Methods The isolation and purification of compounds were performed by means of column chromatography and HPLC. Their structures were elucidated through the analysis of MS, NMR and specific rotation. The antibacterial activities were assayed by Kirby-Bauer test and the broth dilution method. Results From the cultural broth of Aspergillus fumigatus GZWMJZ-152, nine compounds (1~9) were isolated. Their structures were identified as helvolic acid (1), gliotoxin (2), bisdethiobis (methylthio) gliotoxin (3), fumiquinazoline A (4), pseurotin A (5), fumitremorgin C (6), verruculogen (7), fumitremorgin B (8) and fumiquinazoline C (9), respectively. Compounds 1 and 2 showed strong antibacterial activities against MRSA, Vibrio parahaemolyticus, Bacillus cereus, Aeromonas hydrophila, Pseudoalteromonas nigrifaciens and Shewanella marisflavi with minimal inhibitory concentration values from 0.0625 to 2.0μg/mL. Conclusion The antibacterial natural products can be found from cave fungus.
