Abstract：Objective Characterizing the functions of gmrB and gmrA of Micromonospora purpurea G1008 to elucidate the gentamicin biosynthetic mechanisms. Methods The mutant strains GerB102 and GerA102 were constructed by replacing of gmrB and gmrA with the erythromycin resistance gene ermE, respectively. Then the possible alterations of the metabolites in these mutants were analyzed by TLC and MS. The resistance of the mutants was also detected against their own metabolites (gentamicin), respectively. Results The metabolite profile of GerB102 was not significantly changed where gentamicin C components still accumulated, while the metabolite profile of GerA102 was changed. Similar to the native G1008 strain, the tolerance to gentamicin of GerB102 reached 6,000U/mL as well. Comparably, the tolerance to gentamicin of GerA102 was less than 50U/mL. Conclusion The gmrB is neither a key gene for gentamicin biosynthesis nor an essential gene for gentamicin resistance, but the gmrA is a key gene responsible for the resistance to gentamicin.
万云凤 连榕 洪文荣* 石贤爱. 庆大霉素生物合成基因簇中抗性基因gmrB及gmrA的功能研究[J]. 中国抗生素杂志, 2018, 43(6): 688-695.
Wan Yun-feng,Lian Rong,Hong Wen-rong and Shi Xian-ai. Study on the function of resistant genes gmrB and gmrA in gentamicin biosynthetic gene cluster. CJA, 2018, 43(6): 688-695.
