Abstract：Objective To establish an HPLC method for the simultaneous determination of five kinds of antimicrobial agents (methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, mercurothiolate, benzalkonium bromide and benzalkonium chloride) in lomefloxacin hydrochloride eye drops. Methods The analysis was carried out using an extend C18 column (4.6mm×250mm, 5μm). The mobile phase consisted of 1% triethylamine (adjusting pH to 3.0 with phosphoric acid) and methanol as the gradient elute. The flow rate was 1.0mL/min, the column temperature was 35℃ and the detection wavelength was 262nm. Results The five kinds of antimicrobial agents were well separated. The linear range of methyl p-hydroxybenzoate was 0.0410~0.8204mg/mL (r=1.000), and the average recovery was 100.2% (RSD=0.8%, n=9). The linear range of ethyl p-hydroxybenzoate was 0.0400~0.7996mg/mL (r=1.000), and the average recovery was of benzalkonium chloride was 0.0197~0.3932mg/mL (r=1.000), and the average recovery was 100.4% (RSD=1.0%, n=9). Conclusion The method is accurate, sensitive and simple, and it can be used for the determination of methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, mercurothiolate, benzalkonium bromide, benzalkonium chloride in lomefloxacin hydrochloride eye drops. 100.3% (RSD=0.7%, n=9). The linear range of mercurothiolate was 0.0806~1.6112mg/mL (r=0.9995), and the average recovery was 100.8% (RSD=0.9%, n=9). The linear range of benzalkonium bromide was 0.0204~0.4076mg/mL (r=0.9998), and the average recovery was 100.0% (RSD=0.9%, n=9). The linear range
逄焕欢1 高磊1 杨志宏2 冯艳春3,*. 建立HPLC法测定盐酸洛美沙星滴眼液中5种抑菌剂[J]. 中国抗生素杂志, 2018, 43(6): 709-714.
Pang Huan-huan,Gao Lei,Yang Zhi-hong and Feng Yan-chun. Establishment of an HPLC method for the determination of five kinds of antimicrobial agents in lomefloxacin hydrochloride eye drops. CJA, 2018, 43(6): 709-714.
