Abstract：Objective To get an initial understanding of the antibacterial molecular mechanism of pogotone against S. aureus. Methods Drug susceptibility test was performed by the agar dilution method. RNA-seq of S. aureus ATCC25923 treated with pogotone at 1×MIC after one hour was tested. The data were analyzed by KEGG, GO and Heatmap. Results Drug susceptibility test showed that pogotone had obvious antibacterial effects on S. aureus. Treated with pogotone, 225 differentially expressed genes were screened out. The expression of membrane proteins, the synthesis of some proteins and the transport of glucose and polysaccharides were changed. Conclusion Pogotone can reduce the infection caused by S. aureus. In the process of pogostone into the bacterial cell, pogostone may interact with the cell membrane proteins of S. aureus and the cell membrane permeability changed. Therefore, changes in cell osmotic pressure lead to bacterial death. The data provided support for further experiments.
王晓云 陈渝渝 鲍锦库*. 广藿香酮抗金黄色葡萄球菌分子机制的研究[J]. 中国抗生素杂志, 2018, 43(6): 759-764.
Wang Xiao-yun, Chen Yu-yu and Bao Jin-ku. Study on the molecular mechanism of pogotone against Staphylococcus aureus. CJA, 2018, 43(6): 759-764.