Abstract：Objective To investigate the anti-endotoxin, antibacterial and anti-inflammatory effects of Ilicis chinensis folium water decoction (ICFWD) and reveal the pharmacological properties of the clearing-heat and detoxifying effects of Ilicis chinensis folium. Methods The anti-endotoxin effect of ICFWD in vitro was tested using the limulus agglutination assay. Antibacterial effect for the combination of ICFWD in vitro with cefotaxime, levofloxacin and gentamicin against 25 strains including methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), ESBLs-producing Escherichia coli, non-ESBLs-producing Escherichia coli, and Pseudomonas aeruginosa (P. aeruginosa) were also investigated by the agar 2-fold dilution assay. The anti-inflammatory effect of ICFWD was observed by an xylene-induced auricle inflammation model in mice. Results ICFWD showed anti-endotoxin effects at doses ranging from 6.25 to 100mg/mL. The minimum inhibitory concentration of ICFWD against MSSA and MRSA, ESBLs-producing Escherichia coli, non-ESBLs-producing Escherichia coli, as well as P. aeruginosa were 8~33mg/mL, 33~>133mg/mL and 33~133mg/mL, respectively. The combined treatment of ICFWD with cefotaxime, levofloxacin and gentamicin showed additive effects, the fractional inhibitory concentration (FIC) index were 0.5
程强1 周杨杨1 唐炯2,* 孙文霞1 刘昆1 鲁兰1. 四季青水煎液体外抗内毒素、抗菌和体内抗炎作用[J]. 中国抗生素杂志, 2018, 43(6): 765-771.
Cheng Qiang1, Zhou Yang-yang1, Tang Jiong2, Sun Wen-xia1, Liu Kun1 and Lu Lan1. The anti-endotoxin, antibacterial effects in vitro and anti-inflammatory effects in vivo of Ilicis chinensis folium water decoction. CJA, 2018, 43(6): 765-771.
