Abstract：Objective The common system deviations which affect the measuring accuracy of the microbiological assay (the agar diffusion method) were summarized and methods to control them were identified. Methods The cases in the last decade were collected. These cases had a common feature that is a large gap between results of the arbitration or reexamination and the first inspection. We found out the possible common factors for this phenomenon through analysis, and confirmed the causes by a single variable confirmatory experiment. Results The common system deviations that affect the measuring accuracy of the agar diffusion method mainly include the erroneous calculation of the estimated potency and the concentration ratio, the standardization and proficiency in operation, and the mutation of reference culture. Conclusion It is possible to avoid or eliminate these system deviations on the measuring accuracy of the agar diffusion method by means of detailed experimental operations.
常艳. 影响抗生素微生物检定法(管碟法)测定准确性的常见原因分析[J]. 中国抗生素杂志, 2018, 43(7): 875-880.
Chang Yan. Common factors affecting the results' accuracy of the microbiological assay for antibiotics (the agar diffusion method). CJA, 2018, 43(7): 875-880.