Abstract：Objective To study the isolation and biological activity of secondary metabolites from curvularia borreriae HS-FG-237. Methods Compounds were separated and purified by the Diaion HP20 resincolumn, silica gel column, Sephadex LH-20 gel column and semi-preparative HPLC from the fermentation broth. Structures of the compounds were elucidated by modern spectrum technology. Cytotoxicities of these compounds were detected in vitro against the A549, K562 and MDA-MB-231 cell lines by the CCK8 method. Results Twelve known compounds were isolated from the fermentation broth of C. borreriae HS-FG-237, and structures of the compounds were identified as curvalarol A (1), 3β,12β-dihydroxy-4,4,14α-trimethyl-5α-pregna-7,9(11)-diene-20s-carboxylate (2), curvalarol B (3), 23β-Hydroxy-lanost-24-en (4), cyclo-(Gly-Pro) (5), cyclo-(Pho-Thr) (6), cyclo-(Leu-Pro) (7), 4,6,8-trihydroxy-2,3-dihydro-2H-naphthalen-1-one (8), cyclo-(4-hydroxyl-Pro-Leu) (9), ergosterolperoxide (10), 9-β-D-ribofuranosyl adenine (11), cerebroside C (12). Compounds 1~3 and 10 showed cytotoxicity against human cancer A549, K562 and MDA-MB-231 cell lines. Conclusion Cyclic dipeptides compounds are considered as the main metabolites of C. borreriae HS-FG-237. All compounds are isolated from the soil fungus C. borreriae firstly.
薛长艳. 包瑞弯孢菌C. borreriae HS-FG-237次级代谢产物及其生物活性研究[J]. 中国抗生素杂志, 2018, 43(8): 1026-1030.
Xue Chang-yan. Study on the isolation and biological activity of secondary metabolites from Curvularia borreriae HS-FG-237. CJA, 2018, 43(8): 1026-1030.