Abstract：Diarrhea samples from infectious disease hospitals in Beijing from June 2015 to December 2017 were
collected. Diarrhea pathogens were isolated. Antibiotic susceptibility test, analysis of epidemiological characteristics
and antibiotic resistance characteristics of diarrhea pathogens were performed. The practicality of different detection
methods was explored. A total of 235 diarrhea pathogens were isolated from 1,335 samples, with a positive rate of
17.6%. Salmonella positive rate was the highest (53.62%), followed by Vibrio(20.00%). Young people(38.30%),
migrant workers and unemployed people (59.58%) had the highest rates of diarrhea; males were higher than
females(χ2=18.174，P=0). Detection techniques included Microflex LT, VITEK II, and Serological agglutination.
The Microflex LT identification required the least time and cost. The antibiotics with higher resistance rates against
diarrhea pathogens were ampicillin and compound sulfamethoxazole. Imipenem, cefmetazole, ceftriaxone, and levofloxacin had lower resistance rates. Thus, there were many types of diarrheal pathogenic bacteria in Beijing, and
the epidemiological characteristics were clear. It was recommended to use timely and rapid Microflex LT techniques
for the identification of bacteria, supplemented with serological agglutination when necessary, and the monitoring of
the resistance of various pathogens should be strengthened.
