Abstract：Objective To investigate the distribution and the antibiotic resistance of the bacterial isolates from
cerebrospinal fluid during the period from 2016 to 2017 in Western China. Methods Cerebrospinal fluid isolates
were collected from 10 hospitals in Western China. Antimicrobial susceptibility testing was carried out according to aunified protocol using the Kirby-Bauer method or automated systems. Results were analyzed according to the breakpoints
of CLSI 2016. Results 1,234 bacterial isolates and their antibacterial susceptibility data were collected from all the
10 hospitals, which included 697 (56.5% ) Gram-positive bacterial isolates and 537(43.5%) Gram-negative bacterial
isolates. The most frequently bacteria were coagulase-negative Staphylococcus(CNS), A. baumannii, K. pneumoniae,
E. faecium, E. coli, E. faecalis, S. pneumoniae, A. calcoaceticus, P. aeruginosa, and S. aureus. The prevalence of
MRSA in S. aureus and MRCNS in coagulase-negative Staphylococcus was 27.9% and 66.7%, respectively. No
staphylococcal strains were resistant to vancomycin, teicoplanin and linezolid. The prevalence of vancomycin-resistant
strain was 2.2% in E. faecalis and 1.6% in E. faecium. ESBLs-producing strains accounted for 64.2% in E. coli and
58.1% in K. pneumoniae. The carbapenem resistance rate of K. pneumoniae(22.2%~23.6%) was higher than that of E.
coli(3.6%~4.4%). The carbapenem resistance rate of P. aeruginosa was more than 45%, and the resistance rate of A.
baumannii to carbapenes was up to 85%. Conclusion The resistance to the first line antibiotic was common among
all isolates from cerebrospinal fluid. Antimicrobial agents should be used appropriately to reduce the selection pressure.
Hospital infection control measures should be strengthened to prevent the spread of resistant organisms.
