Abstract：Objective To study the antimicrobial resistance, carbapenemase genotypes, and molecular
epidemiology of Acinetobacter baumannii in Anhui province, in order to provide a theoretical basis for the control
of hospital infections. Methods A total of 312 CRAB non-repeated strains were collected from 43 tier 3 level
hospitals in Anhui province from September 2015 to 2016. Only 147 strains of non-repeated CRAB from 12 hospitals
were selected in this study. The minimal inhibitory concentrations (MIC) of 16 antimicrobial agents against 147
Acinetobacter baumannii isolates were determined by the agar dilution method. Carbapenemase resistance genes were
detected by PCR amplification.The genotype was identified by the multilocus sequence typing technique. Evolutionary
diagram was generated with the eBURST software. Results Our results indicated that all the 16 antibiotics showed
high resistance to the 147 A. baumannii isolates which were all multidrug resistant strains. Among the 16 antibiotics,
TGC (9.27%) and MNO (54.26%) showed the lowest resistance. 143 CRAB strains were found to carry blaOXA-23 gene,
one were found to carry blaOXA-24 gene, four were found to carry blaIMP gene. All the 147 CRAB strains were found to carry blaOXA-51 gene and other Carbapenemase genes were not found. The 147 strains of A. baumannii were divided
into 26 ST genotypes, including 13 ST genotypes belonging to complex 92. ST195 had 48 strains, and was the main
epidemiology type in Anhui province. 15 new ST genotypes were found. Conclusion In this study, 147 CRAB in
Anhui province were seriously resistant to clinical antibiotics. Resistance rates were more than 76% except for MNO.
blaOXA-51 is an innate gene that is located on chromosomes, and its detection rate was100%. blaOXA-23 was the main
carbapenemase gene in Anhui province, and its detection rate was 91.15%. CC92 was the main clone group in Anhui
province, which was consistent with the world's popular cloning group. There were phylogenetic relationships among
the 147 strains, suggesting that there might be a horizontal spread trend.
刘舒雅 赵雷 叶英*. 安徽地区耐碳青霉烯鲍曼不动杆菌分子流行病学研究[J]. 中国抗生素杂志, 2018, 43(9): 1151-1155.
Liu Shu-ya,Zhao Lei and Ye Ying. Molecular epidemiology study of carbapenem-resistant Acinetobacter baumannii in
Anhui province. CJA, 2018, 43(9): 1151-1155.
