Abstract： Objective To determine the content of lincomycin in the microplate fermentation broth by
microplate reader. Methods The main influencing factors of the method were optimized and the precision test
was implemented simultaneously. In order to verify the feasibility of this method, the concentration of lincomycin
in 25 kinds of mutant strains after fermentation in 48-well plates were respectively measured by spectrophotometer
and microplate reader. Results It was determined that the appropriate volume of 0.02mol/L palladium chloride
solution was 150μL in the reaction system for the determination of lincomycin, and the appropriate concentration of
hydrochloric acid was 1mol/L. Under this condition, the concentration of lincomycin could be detected by microplate
reader. The data regression showed a strong correlation between these two methods, and the correlation coefficient was
0.9551. Conclusion The experimental results showed that the microplate reader could accurately, quickly, and easily
determine the content of lincomycin in the microplate fermentation broth. It also laid the foundation for the highthroughput selecting and breeding of high-yield lincomycin from Streptomyces lincolnensis.
