Abstract：To establish a method for determination of the release rate of metronidazole buccal tablets, and compare the release curves among different test methods and among different manufacturers. The newly established flow-through cell method, the basket method and the original small vessel method were employed to investigate the dissolution characteristics of all the samples, and the concentrations of each sampling point were determined by HPLC. The dissolution data were used to analyze the similarity of the release curves and statistical significance of Weibull parameters. The linear correlation of metronidazole was observed in the range of 3.012~50.20μg/mL (r=0.9999), and the sample solution was stable in 24h. With the same device, the drug release curves of different companies were not similar. The results of these methods were analyzed by the Weibull equation, and there was no significant difference in the characteristic dissolution parameters of samples from A manufacturer with the basket method and the flow-through method (P>0.05), but there was significant difference in the parameters of samples from B manufacturer (P<0.01). There was significant difference in dissolution parameters among the metronidazole buccal tablets from different manufacturers. These methods including the flow-through cell method and the basket method could both be used to determine the release of metronidazole buccal tablets. This study is of guiding significance to improve the quality control of metronidazole buccal tablets.