Abstract：Objective To fit the growth curves of Staphylococcus aureus under different folds of minimum inhibitory concentration (MIC) values of quinolones and obtain pivotal pharmacodynamic parameters to support the pharmacokinetic-pharmacodynamic(PK/PD) studies on quinolones. Methods Growth curves of Staphylococcus aureus under concentrations of three different quinolones-sitafloxacin, levofloxacin, and moxifloxacin, were obtained under standard procedures. A pharmacokinetic-pharmacodynamic model using nonlinear mixed-effect estimation (NLME) was constructed to fit the curves. Results The pharmacokinetic-pharmacodynamic model was successfully used in the fitting of growth curves of Staphylococcus aureus under different quinolone concentrations. Pivotal pharmacokinetic-pharmacodynamic parameters such as PK/PD index and PK/PD target were obtained. Conclusion The constructed mathematical model could be used for the curve fitting, the obtained PK/PD target were comparable to the results reported in literatures using other methods.