Abstract：Abstract Objective To analyze the genome sequence information of lipstatin high-producing strain S. toxytricini AP617-N12CA, and lay a foundation for further study on the mechanisms of high yield and regulation of lipstatin biosynthesis. Methods Based on single molecule real-time (SMRT) sequencing technology and Illumina HiSeq high-throughput sequencing technology, the whole genome of AP617-N12CA strain was sequenced. Then sequenced reads were assembled, the encoding genes were predicted, the biological functions of the differential genes were annotated, and the secondary metabolite synthetic gene clusters were predicted and analyzed with software. Results The genome size of AP617-N12CA was about 6.99 Mb with an overall G+C content of 73.76%, and 6,134 protein-coding genes were initially annotated. Remarkably, the genome of AP617-N12CA was composed of a single linear chromosome (6.38 Mb) and a single linear plasmid (0.61 Mb). Based on antiSMASH 2.0 analysis, 22 predicted secondary metabolite gene clusters were identified in AP617-N12CA, and the biosynthetic gene cluster for lipstatin was unexpectedly located on the right arm of the linear plasmid. Conclusion In this study, we firstly reported the complete genome sequence map of the strain AP617-N12CA, and firstly reported the presence of linear plasmid in the species of S. toxytricini. Unexpectedly, we found that the lipstatin biosynthetic gene cluster was located on the right arm of the linear plasmid. This study provided basic data for the analysis of the molecular mechanism of high-yield lipstatin in AP617-N12CA strain and subsequent functional genomics research of S. toxytricini.
引用本文:
李辉1 方志锴2 郭霞凌1,*. 利普斯他汀高产菌株毒三素链霉菌AP617-N12CA的全基因组测序与分析[J]. 中国抗生素杂志, 2022, 47(01): 28-34.
Li Hui1, Fang Zhi-kai2, and Guo Xia-ling. Whole-genome sequencing and analysis of high producing strain Streptomyces toxytricini AP617-N12CA. CJA, 2022, 47(01): 28-34.
