Abstract：Abstract Objective To screen fungi producing anti-inflammatory secondary metabolites isolated
from sedimentss collected in Zhangjiang River Estuary Mangrove National Nature Reserves, Fujian province.
Methods The pNFκB-luc and pRL-SV40-C reporter gene plasmids were co-transfected with Raw264.7 cells,
which were stimulated by lipopolysaccharide (LPS) to establish an in vitro inflammation model, and a dual-luciferase
reporter gene screening system was constructed to screen the anti-inflammatory crude extracts produced by mangrove
sediment-derived fungi; effect of crude extracts on Raw264.7 cells viability was measured using CCK-8 assay; the differentiation effect of crude extracts on LPS-stimulated Raw264.7 cells was observed by optical microscope.
Results 5 positive strains were obtained from 102 mangrove sediment-derived fungi, and their fermented crude
extracts significantly inhibited NF-κB signaling pathway activation in LPS-stimulated Raw264.7 cells, strain codes
were H788, H1458, H515, XT20-8 and T2-3'. Conclusion The secondary metabolites produced by 5 mangrove
sediment-derived fungi strains exert anti-inflammatory effects by inhibiting NF-κB signaling pathway, and these active
components deserved studying by natural products approach.
