Abstract：Abstract Objective To study the secondary metabolites produced by marine-derived fungus Aspergillus sp.
HDN19-401 isolated from the Arctic and their biological activities. Methods The fermentation extracts were isolated
and purified by reversed phase silica gel column chromatography, sephadex LH-20 and semi-preparative HPLC and so on. The chemical structures of these compounds were confirmed by ESI-MS and NMR spectral analysis and
comparison with the data of literature. MICs were obtained by the broth microdilution method. Antitumor activity was
studied by the MTT assay. Results and Conclusion Nine compounds were isolated from the extracts of Aspergillus
sp. HDN19-401, and were identified as 6-O-methylaverufin (1), 6, 8-di-O-methylaverufin (2), 6, 8-di-O-methylnidurufin
(3), aversin (4), oxisterigmatocystin A (5), oxisterigmatocystin B (6), fellutamide C (7), notoamides B (8), and
notoamides C (9). Except compound 2, the other compounds were isolated from Arctic fungus for the first time.
Compound 7 showed moderately cytotoxic activity against K562 cell line with an IC50 value of 7.78 μmol/L.
