Abstract：Abstract Objective To study the antibacterial secondary metabolites from the culture broth produced by endophytic fungus Penicillium sp. SYP-F-7610 isolated from the rhizomes of Matteuccia struthiopteris. Methods
The secondary metabolites were isolated and purified using various chromatographic techniques, including
silica gel column chromatography, ODS column chromatography, and semi-preparative high performance liquid
chromatography. Their structures were identified by various spectroscopic data analysis and comparison such as data
from specific rotation, MS, 1H NMR, and 13C NMR. Results Seven compounds were isolated and identified as
penicillium acid (1), methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate (2), orcinol (3), methyl 4-hydroxyphenylacetate
(4), 6-hydroxy-5-methoxy-3-methyl-3,6-dihydro-2H-pyran-4-carboxylic acid (5), chermesinone A (6), and (R)-
mevalonolactone (7). Compound 1 exhibited the inhibitory activities against Escherichia coli CMCC44103, Salmonela
paratyphi B CMCC50094, Bacillus cereus ATCC14579, and Klebsiella pneumoniae CMCC46117 with IC50 values
of 3.19~6.79 μg/mL. Conclusion No previous study regarding the endophytic fungus isolated from Matteuccia sp.
has been reported. The secondary metabolites from the Penicillium sp. SYP-F-7610 isolated from the rhizomes of
Matteuccia struthiopteris possess rich structures and diverse bioactivities, which is worthy of further investigation.
Compound 5 has not been previously reported from the secondary metabolites of Penicillium genus. It is the first
reported that compound 1 displayed the inhibitory activity against Salmonela paratyphi B.
