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�й�������־ 2009, 34(11) 684- DOI:   1001-8689��2009��11-0684-04  ISSN: 0412-1961 CN: 21-1139/TG

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Article by Su, D. G.
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Article by Zhuo, C.
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The analysis on the detection of and Klebsiella pneumoniae KPC type carbapenemase produced by Escherichia coli
1 The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120; 2 Guangzhou Medical College, Guangzhou 510000; 3 Guangzhou Institute of Respiratory Diseases, Guangzhou 510120
Abstract:

Objective To investigate KPC type carbapenemase production in clinical isolates of Escherichia coli and Klebsiella pneumoniae in Guangzhou. Method To screen carbapenems resistance strains of Klebsiella pneumoniae and Escherichia coli with KB disk test,using imipenem,meropenem and ertapenem. To detect KPC-Enzyme with the modified Hodge test and PCR. Result A total of 491 clinical isolates of Escherichia coli and 190 clinical isolates of Klebsiella pneumoniae were collected from Mar 2007 to Oct 2008, and 6 isolates of Escherichia coli and 6 isolates of Klebsiella pneumoniae were resistant to carbapenems. Both Hodge test and KPC encoding genes were negative in all of the 12 isolates Conclusion No KPC-2 produced isolates was detected in this survey according to previous report in China, KPC-2 enzyme was mainly found in limited areas.

Keywords: KPC type carbapenemase   Hodge test   Klebsiella p neumoniae    Escherichia coli    Carbapenem drug  
�ո����� 2009-04-30 �޻�����  ����淢������  
DOI: 1001-8689��2009��11-0684-04
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